Fig. 2. Changes in Rad51c expression on day 4 (initiation phase) of the transformation assay. (A) Relative gene expression of Rad51c assessed by RT-PCR in Balb/c 3T3 cells on day 4 (initiation phase) of the transformation assay. Cells treated with the metal mixture,. Band intensity was normalized against the Control or siPORT (transfection reagent) condition; endogenous control: Hprt1; n=3, mean±s.e., two-tailed unpaired t-test, *p<0.05. (B) Relative protein expression of Rad51c measured by immunoblot in Balb/c 3T3 cells on day 4 (initiation phase) of the transformation assay. Cells treated with the metal mixture,. Band intensity was normalized against the Control or siPORT (transfection reagent) condition; endogenous control: β-tubilun; n=3, mean±s.e., two-tailed unpaired t-test, *p<0.05.